Guangzhou Regenerative Medicine and Health Guangdong Laboratory (GRMH-GDL)
Guangzhou Regenerative Medicine and Health Guangdong Laboratory (GRMH-GDL)
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Cell Reports | Induction of Pluripotent Stem Cells from Mouse Embryonic Fibroblasts by Jdp2-Jhdm1b-Mkk6- Glis1-Nanog-Essrb-Sall4
Release time:2019-06-19

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Prof. Duanqing Pei published a paper on Cell Reports with collaborators.


Reprogramming somatic cells to pluripotency by Oct4, Sox2, Klf4, and Myc represent a paradigm for cell fate determination. Here, we report a combination of Jdp2, Jhdm1b, Mkk6, Glis1, Nanog, Essrb, and Sall4 (7F) that reprogram mouse embryonic fibroblasts or MEFs to chimera competent induced pluripotent stem cells (iPSCs) efficiently. RNA sequencing (RNA-seq) and ATAC-seq reveal distinct mechanisms for 7F induction of pluripotency. Dropout experiments further reveal a highly cooperative process among 7F to dynamically close and open chromatin loci that encode a network of transcription factors to mediate reprogramming. These results establish an alternative paradigm for reprogramming that may be useful for analyzing cell fate control.


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